Objective: To investigate and compare the influence of tobacco use on the frequency of vocal and laryngeal symptoms in female smokers. Methodology: The study was approved by the Ethics and Research Committee (Protocol: 1.526.594). The sample consisted of 54 women who were not professional voice users, divided into two groups: the Smoking Group (SG), which included 27 female smokers aged between 20 and 79 years (mean=46 years, SD=14 years), and the Control Group (CG), which comprised 27 non-smoking women aged between 20 and 80 years (mean=47 years, SD=15 years). All participants completed the Screening Index for Voice Disorder (SIVD) protocol, which evaluates 12 vocal symptoms. They rated the frequency of each symptom as "never", "rarely", "sometimes", or "always". One point was assigned for each response of "sometimes" or "always," and a total score of more than five points indicated a risk for vocal disorders. The results were analyzed descriptively by percentage, mean, and standard deviation, and comparatively between the SG and CG were made using the Mann-Whitney test with a significance level of p<0.05. Results: The majority of the smokers (70.13%, 19/27) scored above the cutoff point of five, in contrast to only 6.45% (2/27) of non-smokers. Regarding the mean and standard deviation (SD) of the scores, the SG had an average score of 5 points (SD=1.74), while the CG had an average score of 2 points (SD=1.68). A comparison between the two groups revealed a significant increase in the self-reported frequency of vocal symptoms (p=0.0013) in the SG compared to the CG. Conclusion: Tobacco use significantly increased the frequency of vocal symptoms in the majority of female smokers, and this increase was statistically significant when compared to non-smokers.